buffer ratio Search Results


giemsa / buffer solution (ratio 3:11)  (Sysmex Corporation)
90
Sysmex Corporation giemsa / buffer solution (ratio 3:11)
ETO2::GLIS2 induces AMKL features in fetal liver and bone marrow, cord blood but not adult HSPCs. A Experimental design to address the impact of the ETO2::GLIS2 ( EG) fusion gene on fetal, early post-natal and adult HSPCs. CD34 + cells were isolated from fetal liver (FL), fetal bone marrow (FBM), cord blood (CB), allogenic bone marrow (ABM) and mobilized peripheral blood (mPB) mononuclear cells. EG expression was induced by lentiviral vector delivery (GFP + cells) or engineered from the endogenous loci by CRISPR/Cas9. Control (CTL) and EG -expressing cells were tested in long-term liquid culture (LT-LC) and colony forming unit assay (CFU), and transcriptomic analysis was performed at different stages of LT-LC. B Cumulative number of total cells (upper panels) and percentage of GFP + cells (lower panels) of CTL and EG -transduced FL ( n = 9 samples, ≥ 3 biological replicates/time point, except %GFP in CTL, 42 days, n = 1), FBM ( n = 4 samples, ≥ 2 biological replicates/time point, except %GFP in CTL, 35 days, n = 1), CB ( n = 9, ≥ 5 biological replicates/time point), ABM ( n = 2, 2 biological replicates/time point, except 7, 28, 42 days, n = 1) and mPB ( n = 2, 2 biological replicates/time point) CD34 + cells during LT-LC. Mean ± SEM of biological replicates is shown. C Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression in EG and CTL GFP + FL, FBM and CB cells at day 21 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells (D) EG fusion frequency assessed by PCR amplification of EG derivative at 2 days (Donor (FL) 1) and 3 days (Donor (FL) 2) after electroporation. PCR were performed in duplicates on DNA dilutions starting from 50 ng DNA (≈ 8 000 cells) to 0.3 ng DNA (≈ 50 cells) . E Cumulative number of CD33 + KIT + cells along culture of CRISPR-edited inv(16) FL CD34 + cells. Mean of technical triplicates is shown for each FL donor (CTL n = 1, EG n = 2). F Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression on CRISPR-edited inv(16) FL EG CD34 + cells at day 40–44 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells <t>(G)</t> <t>May-Grünwald</t> <t>Giemsa</t> stained cytospins from FL cultures (CTL and EG -transduced and EG -engineered cells) at day 20 of LT-LC. Magnification: × 40. H Colony potential assessment upon culture of 500 (P1, CD34 + ) or 50,000 (P2-4) CTL and EG-FL ( n = 8), FBM ( n = 3), CB ( n = 4) and ABM ( n = 2) cells in semi-solid methylcellulose culture medium. Serial replating was performed every 12 to 14 days. P1: first passage, P2: secondary, P3: tertiary, P4: quaternary. Mean ± SEM of biological replicates is shown. Statistical significance is indicated as p values (B and H: Mann–Whitney non parametric test). *: p < 0.05, **: p < 0.01
Giemsa / Buffer Solution (Ratio 3:11), supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/giemsa / buffer solution (ratio 3:11)/product/Sysmex Corporation
Average 90 stars, based on 1 article reviews
giemsa / buffer solution (ratio 3:11) - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
diluted blood in a ratio of 1:6 blood to buffer enlisstii  (Medion Diagnostics)
90
Medion Diagnostics diluted blood in a ratio of 1:6 blood to buffer enlisstii
ETO2::GLIS2 induces AMKL features in fetal liver and bone marrow, cord blood but not adult HSPCs. A Experimental design to address the impact of the ETO2::GLIS2 ( EG) fusion gene on fetal, early post-natal and adult HSPCs. CD34 + cells were isolated from fetal liver (FL), fetal bone marrow (FBM), cord blood (CB), allogenic bone marrow (ABM) and mobilized peripheral blood (mPB) mononuclear cells. EG expression was induced by lentiviral vector delivery (GFP + cells) or engineered from the endogenous loci by CRISPR/Cas9. Control (CTL) and EG -expressing cells were tested in long-term liquid culture (LT-LC) and colony forming unit assay (CFU), and transcriptomic analysis was performed at different stages of LT-LC. B Cumulative number of total cells (upper panels) and percentage of GFP + cells (lower panels) of CTL and EG -transduced FL ( n = 9 samples, ≥ 3 biological replicates/time point, except %GFP in CTL, 42 days, n = 1), FBM ( n = 4 samples, ≥ 2 biological replicates/time point, except %GFP in CTL, 35 days, n = 1), CB ( n = 9, ≥ 5 biological replicates/time point), ABM ( n = 2, 2 biological replicates/time point, except 7, 28, 42 days, n = 1) and mPB ( n = 2, 2 biological replicates/time point) CD34 + cells during LT-LC. Mean ± SEM of biological replicates is shown. C Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression in EG and CTL GFP + FL, FBM and CB cells at day 21 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells (D) EG fusion frequency assessed by PCR amplification of EG derivative at 2 days (Donor (FL) 1) and 3 days (Donor (FL) 2) after electroporation. PCR were performed in duplicates on DNA dilutions starting from 50 ng DNA (≈ 8 000 cells) to 0.3 ng DNA (≈ 50 cells) . E Cumulative number of CD33 + KIT + cells along culture of CRISPR-edited inv(16) FL CD34 + cells. Mean of technical triplicates is shown for each FL donor (CTL n = 1, EG n = 2). F Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression on CRISPR-edited inv(16) FL EG CD34 + cells at day 40–44 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells <t>(G)</t> <t>May-Grünwald</t> <t>Giemsa</t> stained cytospins from FL cultures (CTL and EG -transduced and EG -engineered cells) at day 20 of LT-LC. Magnification: × 40. H Colony potential assessment upon culture of 500 (P1, CD34 + ) or 50,000 (P2-4) CTL and EG-FL ( n = 8), FBM ( n = 3), CB ( n = 4) and ABM ( n = 2) cells in semi-solid methylcellulose culture medium. Serial replating was performed every 12 to 14 days. P1: first passage, P2: secondary, P3: tertiary, P4: quaternary. Mean ± SEM of biological replicates is shown. Statistical significance is indicated as p values (B and H: Mann–Whitney non parametric test). *: p < 0.05, **: p < 0.01
Diluted Blood In A Ratio Of 1:6 Blood To Buffer Enlisstii, supplied by Medion Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/diluted blood in a ratio of 1:6 blood to buffer enlisstii/product/Medion Diagnostics
Average 90 stars, based on 1 article reviews
diluted blood in a ratio of 1:6 blood to buffer enlisstii - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
reservoir buffer (ratio 1:1) containing 100 mm mes ph 5.5, 100 mm kcl, 30% peg400, and 8% polypropylene glycol-p400  (Innovadyne Technologies)
90
Innovadyne Technologies reservoir buffer (ratio 1:1) containing 100 mm mes ph 5.5, 100 mm kcl, 30% peg400, and 8% polypropylene glycol-p400
ETO2::GLIS2 induces AMKL features in fetal liver and bone marrow, cord blood but not adult HSPCs. A Experimental design to address the impact of the ETO2::GLIS2 ( EG) fusion gene on fetal, early post-natal and adult HSPCs. CD34 + cells were isolated from fetal liver (FL), fetal bone marrow (FBM), cord blood (CB), allogenic bone marrow (ABM) and mobilized peripheral blood (mPB) mononuclear cells. EG expression was induced by lentiviral vector delivery (GFP + cells) or engineered from the endogenous loci by CRISPR/Cas9. Control (CTL) and EG -expressing cells were tested in long-term liquid culture (LT-LC) and colony forming unit assay (CFU), and transcriptomic analysis was performed at different stages of LT-LC. B Cumulative number of total cells (upper panels) and percentage of GFP + cells (lower panels) of CTL and EG -transduced FL ( n = 9 samples, ≥ 3 biological replicates/time point, except %GFP in CTL, 42 days, n = 1), FBM ( n = 4 samples, ≥ 2 biological replicates/time point, except %GFP in CTL, 35 days, n = 1), CB ( n = 9, ≥ 5 biological replicates/time point), ABM ( n = 2, 2 biological replicates/time point, except 7, 28, 42 days, n = 1) and mPB ( n = 2, 2 biological replicates/time point) CD34 + cells during LT-LC. Mean ± SEM of biological replicates is shown. C Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression in EG and CTL GFP + FL, FBM and CB cells at day 21 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells (D) EG fusion frequency assessed by PCR amplification of EG derivative at 2 days (Donor (FL) 1) and 3 days (Donor (FL) 2) after electroporation. PCR were performed in duplicates on DNA dilutions starting from 50 ng DNA (≈ 8 000 cells) to 0.3 ng DNA (≈ 50 cells) . E Cumulative number of CD33 + KIT + cells along culture of CRISPR-edited inv(16) FL CD34 + cells. Mean of technical triplicates is shown for each FL donor (CTL n = 1, EG n = 2). F Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression on CRISPR-edited inv(16) FL EG CD34 + cells at day 40–44 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells <t>(G)</t> <t>May-Grünwald</t> <t>Giemsa</t> stained cytospins from FL cultures (CTL and EG -transduced and EG -engineered cells) at day 20 of LT-LC. Magnification: × 40. H Colony potential assessment upon culture of 500 (P1, CD34 + ) or 50,000 (P2-4) CTL and EG-FL ( n = 8), FBM ( n = 3), CB ( n = 4) and ABM ( n = 2) cells in semi-solid methylcellulose culture medium. Serial replating was performed every 12 to 14 days. P1: first passage, P2: secondary, P3: tertiary, P4: quaternary. Mean ± SEM of biological replicates is shown. Statistical significance is indicated as p values (B and H: Mann–Whitney non parametric test). *: p < 0.05, **: p < 0.01
Reservoir Buffer (Ratio 1:1) Containing 100 Mm Mes Ph 5.5, 100 Mm Kcl, 30% Peg400, And 8% Polypropylene Glycol P400, supplied by Innovadyne Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reservoir buffer (ratio 1:1) containing 100 mm mes ph 5.5, 100 mm kcl, 30% peg400, and 8% polypropylene glycol-p400/product/Innovadyne Technologies
Average 90 stars, based on 1 article reviews
reservoir buffer (ratio 1:1) containing 100 mm mes ph 5.5, 100 mm kcl, 30% peg400, and 8% polypropylene glycol-p400 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
anaerobic phosphate buffer (0.1m of nah 2 po 4 and 0.1m of na 2 hpo 4 in 2:1 ratio) containing 10% glycerol  (Coy Laboratory)
90
Coy Laboratory anaerobic phosphate buffer (0.1m of nah 2 po 4 and 0.1m of na 2 hpo 4 in 2:1 ratio) containing 10% glycerol
ETO2::GLIS2 induces AMKL features in fetal liver and bone marrow, cord blood but not adult HSPCs. A Experimental design to address the impact of the ETO2::GLIS2 ( EG) fusion gene on fetal, early post-natal and adult HSPCs. CD34 + cells were isolated from fetal liver (FL), fetal bone marrow (FBM), cord blood (CB), allogenic bone marrow (ABM) and mobilized peripheral blood (mPB) mononuclear cells. EG expression was induced by lentiviral vector delivery (GFP + cells) or engineered from the endogenous loci by CRISPR/Cas9. Control (CTL) and EG -expressing cells were tested in long-term liquid culture (LT-LC) and colony forming unit assay (CFU), and transcriptomic analysis was performed at different stages of LT-LC. B Cumulative number of total cells (upper panels) and percentage of GFP + cells (lower panels) of CTL and EG -transduced FL ( n = 9 samples, ≥ 3 biological replicates/time point, except %GFP in CTL, 42 days, n = 1), FBM ( n = 4 samples, ≥ 2 biological replicates/time point, except %GFP in CTL, 35 days, n = 1), CB ( n = 9, ≥ 5 biological replicates/time point), ABM ( n = 2, 2 biological replicates/time point, except 7, 28, 42 days, n = 1) and mPB ( n = 2, 2 biological replicates/time point) CD34 + cells during LT-LC. Mean ± SEM of biological replicates is shown. C Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression in EG and CTL GFP + FL, FBM and CB cells at day 21 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells (D) EG fusion frequency assessed by PCR amplification of EG derivative at 2 days (Donor (FL) 1) and 3 days (Donor (FL) 2) after electroporation. PCR were performed in duplicates on DNA dilutions starting from 50 ng DNA (≈ 8 000 cells) to 0.3 ng DNA (≈ 50 cells) . E Cumulative number of CD33 + KIT + cells along culture of CRISPR-edited inv(16) FL CD34 + cells. Mean of technical triplicates is shown for each FL donor (CTL n = 1, EG n = 2). F Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression on CRISPR-edited inv(16) FL EG CD34 + cells at day 40–44 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells <t>(G)</t> <t>May-Grünwald</t> <t>Giemsa</t> stained cytospins from FL cultures (CTL and EG -transduced and EG -engineered cells) at day 20 of LT-LC. Magnification: × 40. H Colony potential assessment upon culture of 500 (P1, CD34 + ) or 50,000 (P2-4) CTL and EG-FL ( n = 8), FBM ( n = 3), CB ( n = 4) and ABM ( n = 2) cells in semi-solid methylcellulose culture medium. Serial replating was performed every 12 to 14 days. P1: first passage, P2: secondary, P3: tertiary, P4: quaternary. Mean ± SEM of biological replicates is shown. Statistical significance is indicated as p values (B and H: Mann–Whitney non parametric test). *: p < 0.05, **: p < 0.01
Anaerobic Phosphate Buffer (0.1m Of Nah 2 Po 4 And 0.1m Of Na 2 Hpo 4 In 2:1 Ratio) Containing 10% Glycerol, supplied by Coy Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anaerobic phosphate buffer (0.1m of nah 2 po 4 and 0.1m of na 2 hpo 4 in 2:1 ratio) containing 10% glycerol/product/Coy Laboratory
Average 90 stars, based on 1 article reviews
anaerobic phosphate buffer (0.1m of nah 2 po 4 and 0.1m of na 2 hpo 4 in 2:1 ratio) containing 10% glycerol - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
0.2 m na2hpo4-nah2po4 buffer (ph 8) at a ratio of 1:15 (w/v)  (Omni International)
90
Omni International 0.2 m na2hpo4-nah2po4 buffer (ph 8) at a ratio of 1:15 (w/v)
ETO2::GLIS2 induces AMKL features in fetal liver and bone marrow, cord blood but not adult HSPCs. A Experimental design to address the impact of the ETO2::GLIS2 ( EG) fusion gene on fetal, early post-natal and adult HSPCs. CD34 + cells were isolated from fetal liver (FL), fetal bone marrow (FBM), cord blood (CB), allogenic bone marrow (ABM) and mobilized peripheral blood (mPB) mononuclear cells. EG expression was induced by lentiviral vector delivery (GFP + cells) or engineered from the endogenous loci by CRISPR/Cas9. Control (CTL) and EG -expressing cells were tested in long-term liquid culture (LT-LC) and colony forming unit assay (CFU), and transcriptomic analysis was performed at different stages of LT-LC. B Cumulative number of total cells (upper panels) and percentage of GFP + cells (lower panels) of CTL and EG -transduced FL ( n = 9 samples, ≥ 3 biological replicates/time point, except %GFP in CTL, 42 days, n = 1), FBM ( n = 4 samples, ≥ 2 biological replicates/time point, except %GFP in CTL, 35 days, n = 1), CB ( n = 9, ≥ 5 biological replicates/time point), ABM ( n = 2, 2 biological replicates/time point, except 7, 28, 42 days, n = 1) and mPB ( n = 2, 2 biological replicates/time point) CD34 + cells during LT-LC. Mean ± SEM of biological replicates is shown. C Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression in EG and CTL GFP + FL, FBM and CB cells at day 21 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells (D) EG fusion frequency assessed by PCR amplification of EG derivative at 2 days (Donor (FL) 1) and 3 days (Donor (FL) 2) after electroporation. PCR were performed in duplicates on DNA dilutions starting from 50 ng DNA (≈ 8 000 cells) to 0.3 ng DNA (≈ 50 cells) . E Cumulative number of CD33 + KIT + cells along culture of CRISPR-edited inv(16) FL CD34 + cells. Mean of technical triplicates is shown for each FL donor (CTL n = 1, EG n = 2). F Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression on CRISPR-edited inv(16) FL EG CD34 + cells at day 40–44 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells <t>(G)</t> <t>May-Grünwald</t> <t>Giemsa</t> stained cytospins from FL cultures (CTL and EG -transduced and EG -engineered cells) at day 20 of LT-LC. Magnification: × 40. H Colony potential assessment upon culture of 500 (P1, CD34 + ) or 50,000 (P2-4) CTL and EG-FL ( n = 8), FBM ( n = 3), CB ( n = 4) and ABM ( n = 2) cells in semi-solid methylcellulose culture medium. Serial replating was performed every 12 to 14 days. P1: first passage, P2: secondary, P3: tertiary, P4: quaternary. Mean ± SEM of biological replicates is shown. Statistical significance is indicated as p values (B and H: Mann–Whitney non parametric test). *: p < 0.05, **: p < 0.01
0.2 M Na2hpo4 Nah2po4 Buffer (Ph 8) At A Ratio Of 1:15 (W/V), supplied by Omni International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/0.2 m na2hpo4-nah2po4 buffer (ph 8) at a ratio of 1:15 (w/v)/product/Omni International
Average 90 stars, based on 1 article reviews
0.2 m na2hpo4-nah2po4 buffer (ph 8) at a ratio of 1:15 (w/v) - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
phosphate buffer (nah2po4æ2h2o : na2hpo4æ12h2o, ratio 25 : 4  (FUJIFILM)
90
FUJIFILM phosphate buffer (nah2po4æ2h2o : na2hpo4æ12h2o, ratio 25 : 4
ETO2::GLIS2 induces AMKL features in fetal liver and bone marrow, cord blood but not adult HSPCs. A Experimental design to address the impact of the ETO2::GLIS2 ( EG) fusion gene on fetal, early post-natal and adult HSPCs. CD34 + cells were isolated from fetal liver (FL), fetal bone marrow (FBM), cord blood (CB), allogenic bone marrow (ABM) and mobilized peripheral blood (mPB) mononuclear cells. EG expression was induced by lentiviral vector delivery (GFP + cells) or engineered from the endogenous loci by CRISPR/Cas9. Control (CTL) and EG -expressing cells were tested in long-term liquid culture (LT-LC) and colony forming unit assay (CFU), and transcriptomic analysis was performed at different stages of LT-LC. B Cumulative number of total cells (upper panels) and percentage of GFP + cells (lower panels) of CTL and EG -transduced FL ( n = 9 samples, ≥ 3 biological replicates/time point, except %GFP in CTL, 42 days, n = 1), FBM ( n = 4 samples, ≥ 2 biological replicates/time point, except %GFP in CTL, 35 days, n = 1), CB ( n = 9, ≥ 5 biological replicates/time point), ABM ( n = 2, 2 biological replicates/time point, except 7, 28, 42 days, n = 1) and mPB ( n = 2, 2 biological replicates/time point) CD34 + cells during LT-LC. Mean ± SEM of biological replicates is shown. C Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression in EG and CTL GFP + FL, FBM and CB cells at day 21 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells (D) EG fusion frequency assessed by PCR amplification of EG derivative at 2 days (Donor (FL) 1) and 3 days (Donor (FL) 2) after electroporation. PCR were performed in duplicates on DNA dilutions starting from 50 ng DNA (≈ 8 000 cells) to 0.3 ng DNA (≈ 50 cells) . E Cumulative number of CD33 + KIT + cells along culture of CRISPR-edited inv(16) FL CD34 + cells. Mean of technical triplicates is shown for each FL donor (CTL n = 1, EG n = 2). F Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression on CRISPR-edited inv(16) FL EG CD34 + cells at day 40–44 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells <t>(G)</t> <t>May-Grünwald</t> <t>Giemsa</t> stained cytospins from FL cultures (CTL and EG -transduced and EG -engineered cells) at day 20 of LT-LC. Magnification: × 40. H Colony potential assessment upon culture of 500 (P1, CD34 + ) or 50,000 (P2-4) CTL and EG-FL ( n = 8), FBM ( n = 3), CB ( n = 4) and ABM ( n = 2) cells in semi-solid methylcellulose culture medium. Serial replating was performed every 12 to 14 days. P1: first passage, P2: secondary, P3: tertiary, P4: quaternary. Mean ± SEM of biological replicates is shown. Statistical significance is indicated as p values (B and H: Mann–Whitney non parametric test). *: p < 0.05, **: p < 0.01
Phosphate Buffer (Nah2po4æ2h2o : Na2hpo4æ12h2o, Ratio 25 : 4, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphate buffer (nah2po4æ2h2o : na2hpo4æ12h2o, ratio 25 : 4/product/FUJIFILM
Average 90 stars, based on 1 article reviews
phosphate buffer (nah2po4æ2h2o : na2hpo4æ12h2o, ratio 25 : 4 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
working buffer prepared by propidium iodide and rnase in a 9:1 ratio  (Keygen Biotech)
90
Keygen Biotech working buffer prepared by propidium iodide and rnase in a 9:1 ratio
ETO2::GLIS2 induces AMKL features in fetal liver and bone marrow, cord blood but not adult HSPCs. A Experimental design to address the impact of the ETO2::GLIS2 ( EG) fusion gene on fetal, early post-natal and adult HSPCs. CD34 + cells were isolated from fetal liver (FL), fetal bone marrow (FBM), cord blood (CB), allogenic bone marrow (ABM) and mobilized peripheral blood (mPB) mononuclear cells. EG expression was induced by lentiviral vector delivery (GFP + cells) or engineered from the endogenous loci by CRISPR/Cas9. Control (CTL) and EG -expressing cells were tested in long-term liquid culture (LT-LC) and colony forming unit assay (CFU), and transcriptomic analysis was performed at different stages of LT-LC. B Cumulative number of total cells (upper panels) and percentage of GFP + cells (lower panels) of CTL and EG -transduced FL ( n = 9 samples, ≥ 3 biological replicates/time point, except %GFP in CTL, 42 days, n = 1), FBM ( n = 4 samples, ≥ 2 biological replicates/time point, except %GFP in CTL, 35 days, n = 1), CB ( n = 9, ≥ 5 biological replicates/time point), ABM ( n = 2, 2 biological replicates/time point, except 7, 28, 42 days, n = 1) and mPB ( n = 2, 2 biological replicates/time point) CD34 + cells during LT-LC. Mean ± SEM of biological replicates is shown. C Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression in EG and CTL GFP + FL, FBM and CB cells at day 21 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells (D) EG fusion frequency assessed by PCR amplification of EG derivative at 2 days (Donor (FL) 1) and 3 days (Donor (FL) 2) after electroporation. PCR were performed in duplicates on DNA dilutions starting from 50 ng DNA (≈ 8 000 cells) to 0.3 ng DNA (≈ 50 cells) . E Cumulative number of CD33 + KIT + cells along culture of CRISPR-edited inv(16) FL CD34 + cells. Mean of technical triplicates is shown for each FL donor (CTL n = 1, EG n = 2). F Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression on CRISPR-edited inv(16) FL EG CD34 + cells at day 40–44 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells <t>(G)</t> <t>May-Grünwald</t> <t>Giemsa</t> stained cytospins from FL cultures (CTL and EG -transduced and EG -engineered cells) at day 20 of LT-LC. Magnification: × 40. H Colony potential assessment upon culture of 500 (P1, CD34 + ) or 50,000 (P2-4) CTL and EG-FL ( n = 8), FBM ( n = 3), CB ( n = 4) and ABM ( n = 2) cells in semi-solid methylcellulose culture medium. Serial replating was performed every 12 to 14 days. P1: first passage, P2: secondary, P3: tertiary, P4: quaternary. Mean ± SEM of biological replicates is shown. Statistical significance is indicated as p values (B and H: Mann–Whitney non parametric test). *: p < 0.05, **: p < 0.01
Working Buffer Prepared By Propidium Iodide And Rnase In A 9:1 Ratio, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/working buffer prepared by propidium iodide and rnase in a 9:1 ratio/product/Keygen Biotech
Average 90 stars, based on 1 article reviews
working buffer prepared by propidium iodide and rnase in a 9:1 ratio - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


ETO2::GLIS2 induces AMKL features in fetal liver and bone marrow, cord blood but not adult HSPCs. A Experimental design to address the impact of the ETO2::GLIS2 ( EG) fusion gene on fetal, early post-natal and adult HSPCs. CD34 + cells were isolated from fetal liver (FL), fetal bone marrow (FBM), cord blood (CB), allogenic bone marrow (ABM) and mobilized peripheral blood (mPB) mononuclear cells. EG expression was induced by lentiviral vector delivery (GFP + cells) or engineered from the endogenous loci by CRISPR/Cas9. Control (CTL) and EG -expressing cells were tested in long-term liquid culture (LT-LC) and colony forming unit assay (CFU), and transcriptomic analysis was performed at different stages of LT-LC. B Cumulative number of total cells (upper panels) and percentage of GFP + cells (lower panels) of CTL and EG -transduced FL ( n = 9 samples, ≥ 3 biological replicates/time point, except %GFP in CTL, 42 days, n = 1), FBM ( n = 4 samples, ≥ 2 biological replicates/time point, except %GFP in CTL, 35 days, n = 1), CB ( n = 9, ≥ 5 biological replicates/time point), ABM ( n = 2, 2 biological replicates/time point, except 7, 28, 42 days, n = 1) and mPB ( n = 2, 2 biological replicates/time point) CD34 + cells during LT-LC. Mean ± SEM of biological replicates is shown. C Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression in EG and CTL GFP + FL, FBM and CB cells at day 21 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells (D) EG fusion frequency assessed by PCR amplification of EG derivative at 2 days (Donor (FL) 1) and 3 days (Donor (FL) 2) after electroporation. PCR were performed in duplicates on DNA dilutions starting from 50 ng DNA (≈ 8 000 cells) to 0.3 ng DNA (≈ 50 cells) . E Cumulative number of CD33 + KIT + cells along culture of CRISPR-edited inv(16) FL CD34 + cells. Mean of technical triplicates is shown for each FL donor (CTL n = 1, EG n = 2). F Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression on CRISPR-edited inv(16) FL EG CD34 + cells at day 40–44 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells (G) May-Grünwald Giemsa stained cytospins from FL cultures (CTL and EG -transduced and EG -engineered cells) at day 20 of LT-LC. Magnification: × 40. H Colony potential assessment upon culture of 500 (P1, CD34 + ) or 50,000 (P2-4) CTL and EG-FL ( n = 8), FBM ( n = 3), CB ( n = 4) and ABM ( n = 2) cells in semi-solid methylcellulose culture medium. Serial replating was performed every 12 to 14 days. P1: first passage, P2: secondary, P3: tertiary, P4: quaternary. Mean ± SEM of biological replicates is shown. Statistical significance is indicated as p values (B and H: Mann–Whitney non parametric test). *: p < 0.05, **: p < 0.01

Journal: Molecular Cancer

Article Title: Developmental interplay between transcriptional alterations and a targetable cytokine signaling dependency in pediatric ETO2::GLIS2 leukemia

doi: 10.1186/s12943-024-02110-y

Figure Lengend Snippet: ETO2::GLIS2 induces AMKL features in fetal liver and bone marrow, cord blood but not adult HSPCs. A Experimental design to address the impact of the ETO2::GLIS2 ( EG) fusion gene on fetal, early post-natal and adult HSPCs. CD34 + cells were isolated from fetal liver (FL), fetal bone marrow (FBM), cord blood (CB), allogenic bone marrow (ABM) and mobilized peripheral blood (mPB) mononuclear cells. EG expression was induced by lentiviral vector delivery (GFP + cells) or engineered from the endogenous loci by CRISPR/Cas9. Control (CTL) and EG -expressing cells were tested in long-term liquid culture (LT-LC) and colony forming unit assay (CFU), and transcriptomic analysis was performed at different stages of LT-LC. B Cumulative number of total cells (upper panels) and percentage of GFP + cells (lower panels) of CTL and EG -transduced FL ( n = 9 samples, ≥ 3 biological replicates/time point, except %GFP in CTL, 42 days, n = 1), FBM ( n = 4 samples, ≥ 2 biological replicates/time point, except %GFP in CTL, 35 days, n = 1), CB ( n = 9, ≥ 5 biological replicates/time point), ABM ( n = 2, 2 biological replicates/time point, except 7, 28, 42 days, n = 1) and mPB ( n = 2, 2 biological replicates/time point) CD34 + cells during LT-LC. Mean ± SEM of biological replicates is shown. C Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression in EG and CTL GFP + FL, FBM and CB cells at day 21 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells (D) EG fusion frequency assessed by PCR amplification of EG derivative at 2 days (Donor (FL) 1) and 3 days (Donor (FL) 2) after electroporation. PCR were performed in duplicates on DNA dilutions starting from 50 ng DNA (≈ 8 000 cells) to 0.3 ng DNA (≈ 50 cells) . E Cumulative number of CD33 + KIT + cells along culture of CRISPR-edited inv(16) FL CD34 + cells. Mean of technical triplicates is shown for each FL donor (CTL n = 1, EG n = 2). F Representative flow cytometry analysis of CD33, KIT, CD56 and CD41 expression on CRISPR-edited inv(16) FL EG CD34 + cells at day 40–44 of LT-LC. CD56 and CD41 expression are gated in CD33 + KIT + cells (G) May-Grünwald Giemsa stained cytospins from FL cultures (CTL and EG -transduced and EG -engineered cells) at day 20 of LT-LC. Magnification: × 40. H Colony potential assessment upon culture of 500 (P1, CD34 + ) or 50,000 (P2-4) CTL and EG-FL ( n = 8), FBM ( n = 3), CB ( n = 4) and ABM ( n = 2) cells in semi-solid methylcellulose culture medium. Serial replating was performed every 12 to 14 days. P1: first passage, P2: secondary, P3: tertiary, P4: quaternary. Mean ± SEM of biological replicates is shown. Statistical significance is indicated as p values (B and H: Mann–Whitney non parametric test). *: p < 0.05, **: p < 0.01

Article Snippet: Briefly, slides were subjected to 2 min of a May-Grünwald solution bath, 2 min of a May-Grünwald / Buffer Solution (ratio 1:1) bath, 14 min of a Giemsa / Buffer Solution (ratio 3:11) bath and 1 min of the Buffer Solution bath (all solutions from Sysmex), before drying and covering with a cover-slip.

Techniques: Isolation, Expressing, Plasmid Preparation, CRISPR, Control, Colony-forming Unit Assay, Flow Cytometry, Amplification, Electroporation, Staining, MANN-WHITNEY